affi prep protein a agarose beads (Bio-Rad)
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affi prep protein a agarose beads
Affi Prep Protein A Agarose Beads, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 278 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/affi+prep+protein+a+agarose+beads/Affi-Prep+Protein+A+Support/pm39893637-337-13-19
Average 93 stars, based on 278 article reviews
Affi Prep Protein A Agarose Beads, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 278 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/affi+prep+protein+a+agarose+beads/Affi-Prep+Protein+A+Support/pm39893637-337-13-19
Average 93 stars, based on 278 article reviews
affi prep protein a agarose beads - by Bioz Stars,
2026-09
93/100 stars
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Immunoprecipitation:Article Title: On the regulation, function, and localization of the DNA-dependent ATPase PICH Article Snippet: Preparation of lysates and western blot analyses were performed as described previously (Sillje et al. ) using Tris lysis buffer (50 mM Tris–HCl pH 7.5, 150 mM NaCl, 1 % IGEPAL CA-630) containing 20 mM NaF, 20 mM β-glycerophosphate, 0.3 mM Na-vanadate, 20 μg/ml RNase A, 20 μg/ml DNase supplemented fresh with 1 mM Pefabloc SC (Roth), and 1/100 phosphatase inhibitor cocktail 1 + 2 (Sigma-Aldrich, St. Louise, MO, USA). .. Immunoprecipitation of overexpressed PICH was carried out using 0.5 μg of corresponding antibody and 20 μL Article Title: The homologous recombination factors BRCA2 and PALB2 interplay with mismatch repair pathways to maintain centromere stability and cell viability. Article Snippet: Primary and secondary antibodies (key resources table) were diluted in Blocking buffer and applied for 1 h at room temperature or overnight at 4 C. Membranes were washed in PBS with 0.05% Tween 20 buffer three times for 5 min. Proteins were detected using 1:1 ECL Western Blotting detection solution (RPN2106, Amersham) for 30 s and developed using chemiluminescence film (28906837, Cytiva) in a Xograph developer, as required. .. Immunoprecipitation (IP) IgG (02–6502, Invitrogen) or BRCA2 antibodies (OP95, Calbiochem) were conjugated to Lysis:Article Title: On the regulation, function, and localization of the DNA-dependent ATPase PICH Article Snippet: Preparation of lysates and western blot analyses were performed as described previously (Sillje et al. ) using Tris lysis buffer (50 mM Tris–HCl pH 7.5, 150 mM NaCl, 1 % IGEPAL CA-630) containing 20 mM NaF, 20 mM β-glycerophosphate, 0.3 mM Na-vanadate, 20 μg/ml RNase A, 20 μg/ml DNase supplemented fresh with 1 mM Pefabloc SC (Roth), and 1/100 phosphatase inhibitor cocktail 1 + 2 (Sigma-Aldrich, St. Louise, MO, USA). .. Immunoprecipitation of overexpressed PICH was carried out using 0.5 μg of corresponding antibody and 20 μL other:Article Title: The homologous recombination factors BRCA2 and PALB2 interplay with mismatch repair pathways to maintain centromere stability and cell viability. Article Snippet: The beads were washed twice with PBS before incubating with the relevant antibody (2 mL antibody per 5 mL beads) on a rotor wheel at room temperature for 3 h. Antibody-crosslinked beads were pelleted (800 x g, 4 min) and washed twice in coupling buffer (27 mM sodium tetraborate (221732, Sigma-Aldrich), 73 mM boric acid (B7660, SigmaAldrich)), once in DIM-PIM buffer (56 mM dimethyl pimelimidate dihydrochloride (D8388, Sigma-Aldrich), 0.1 M sodium tetraborate), and then incubated in DIM-PIM buffer overnight, 4 C. Beads were subsequently washed thrice in coupling buffer and once in 1M Tris pH 9. |